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pcdna3 1 stim1 venus 173 c  (Addgene inc)


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    Structured Review

    Addgene inc pcdna3 1 stim1 venus 173 c
    Pcdna3 1 Stim1 Venus 173 C, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna3+1+stim1+venus+173+c/pcDNA3%2E1-STIM1-Venus-173-C+(Plasmid+%2387619)/pmc13034653-68-0-2
    Average 94 stars, based on 4 article reviews
    pcdna3 1 stim1 venus 173 c - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Cloning:

    Article Title: A shear stress-responsive pathway in monocytes drives cardiopulmonary bypass-induced inflammation via spectrin/RAF1/store-operated calcium entry
    Article Snippet: Enhanced GFP (EGFP) was PCR cloned from pLenti CMV GFP Puro (Addgene 658–5) with primers CTCGTCGACCATGGTGAGCAAGGG and CTCGGATCC CGTTACTTGTACAGCTCGT and cloned into hIL8 -pCHD at the SalI and BamHI restriction sites. .. Cloning for bimolecular fluorescence complementation (BiFC) of store operated calcium entry (SOCE) genes lentivirus constructs: N-terminal Venus’s fragment fused to full-length ORAI1 and C-terminal Venus fused to full-length STIM1 were cut out and amplified from plasmids pcDNA3-Venus-173-N-ORAI1 (a gift from Jin Zhang; Addgene plasmid #87618) and pcDNA3.1-STIM1-Venus-173-C (a gift from Jin Zhang; Addgene plasmid #87619), respectively. .. These two inserts were separately cloned into the pCDH-CMV-Nluc plasmid (a gift from Kazuhiro Oka; Addgene plasmid #73038) in place of the Nanoluciferase (Nluc) reporter using BamH1 and EcoR1 restriction sites to yield two distinct plasmids, each containing a unique fragment for BiFC.

    Article Title: A shear stress-responsive pathway in monocytes drives cardiopulmonary bypass-induced inflammation via spectrin/RAF1/store-operated calcium entry.
    Article Snippet: Enhanced GFP (EGFP) was PCR cloned from pLenti CMV GFP Puro (Addgene 658-5) with primers CTCGTCGACCATGGTGAGCAAGGG and CTCGGATCC CGTTACTTGTACAGCTCGT and cloned into hIL8-pCHD at the SalI and BamHI restriction sites. .. Cloning for bimolecular fluorescence complementation (BiFC) of store operated calcium entry (SOCE) genes lentivirus constructs: N-terminal Venus’s fragment fused to full-length ORAI1 and C-terminal Venus fused to full-length STIM1 were cut out and amplified from plasmids pcDNA3-Venus-173-N-ORAI1 (a gift from Jin Zhang; Addgene plasmid #87618) and pcDNA3.1-STIM1-Venus-173-C (a gift from Jin Zhang; Addgene plasmid #87619), respectively. .. These two inserts were separately cloned into the pCDH-CMV-Nluc plasmid (a gift from Kazuhiro Oka; Addgene plasmid #73038) in place of the Nanoluciferase (Nluc) reporter using BamH1 and EcoR1 restriction sites to yield two distinct plasmids, each containing a unique fragment for BiFC.

    Article Title: Hemodynamic stress activates inflammatory responses and cell death through spectrin-dependent modulation of Store Operated Calcium Entry
    Article Snippet: Enhanced GFP (EGFP ) was PCR cloned from pLenti CMV GFP Puro (Addgene 658-5) with primers CTCGTCGACCATGGTGAGCAAGGG and CTCGGATCC CGTTACTTGTACAGCTCGT and cloned into hIL8 -pCHD at the SalI and BamHI restriction sites. .. Cloning for bimolecular fluorescence complementation (BiFC) of store operated calcium entry (SOCE) genes lentivirus constructs: N-terminal Venus fragment fused to full-length ORAI1 and C-terminal Venus fused to full-length STIM1 were cut out and amplified from plasmids pcDNA3-Venus-173-N-ORAI1 (a gift from Jin Zhang; Addgene plasmid #87618) and pcDNA3.1-STIM1-Venus-173-C (a gift from Jin Zhang; Addgene plasmid #87619), respectively. .. These two inserts were separately cloned into the pCDH-CMV-Nluc plasmid (a gift from Kazuhiro Oka; Addgene plasmid #73038) in place of the Nanoluciferase (Nluc) reporter using BamH1 and EcoR1 restriction sites to yield two distinct plasmids, each containing a unique fragment for BiFC.

    Fluorescence:

    Article Title: A shear stress-responsive pathway in monocytes drives cardiopulmonary bypass-induced inflammation via spectrin/RAF1/store-operated calcium entry
    Article Snippet: Enhanced GFP (EGFP) was PCR cloned from pLenti CMV GFP Puro (Addgene 658–5) with primers CTCGTCGACCATGGTGAGCAAGGG and CTCGGATCC CGTTACTTGTACAGCTCGT and cloned into hIL8 -pCHD at the SalI and BamHI restriction sites. .. Cloning for bimolecular fluorescence complementation (BiFC) of store operated calcium entry (SOCE) genes lentivirus constructs: N-terminal Venus’s fragment fused to full-length ORAI1 and C-terminal Venus fused to full-length STIM1 were cut out and amplified from plasmids pcDNA3-Venus-173-N-ORAI1 (a gift from Jin Zhang; Addgene plasmid #87618) and pcDNA3.1-STIM1-Venus-173-C (a gift from Jin Zhang; Addgene plasmid #87619), respectively. .. These two inserts were separately cloned into the pCDH-CMV-Nluc plasmid (a gift from Kazuhiro Oka; Addgene plasmid #73038) in place of the Nanoluciferase (Nluc) reporter using BamH1 and EcoR1 restriction sites to yield two distinct plasmids, each containing a unique fragment for BiFC.

    Article Title: A shear stress-responsive pathway in monocytes drives cardiopulmonary bypass-induced inflammation via spectrin/RAF1/store-operated calcium entry.
    Article Snippet: Enhanced GFP (EGFP) was PCR cloned from pLenti CMV GFP Puro (Addgene 658-5) with primers CTCGTCGACCATGGTGAGCAAGGG and CTCGGATCC CGTTACTTGTACAGCTCGT and cloned into hIL8-pCHD at the SalI and BamHI restriction sites. .. Cloning for bimolecular fluorescence complementation (BiFC) of store operated calcium entry (SOCE) genes lentivirus constructs: N-terminal Venus’s fragment fused to full-length ORAI1 and C-terminal Venus fused to full-length STIM1 were cut out and amplified from plasmids pcDNA3-Venus-173-N-ORAI1 (a gift from Jin Zhang; Addgene plasmid #87618) and pcDNA3.1-STIM1-Venus-173-C (a gift from Jin Zhang; Addgene plasmid #87619), respectively. .. These two inserts were separately cloned into the pCDH-CMV-Nluc plasmid (a gift from Kazuhiro Oka; Addgene plasmid #73038) in place of the Nanoluciferase (Nluc) reporter using BamH1 and EcoR1 restriction sites to yield two distinct plasmids, each containing a unique fragment for BiFC.

    Article Title: Hemodynamic stress activates inflammatory responses and cell death through spectrin-dependent modulation of Store Operated Calcium Entry
    Article Snippet: Enhanced GFP (EGFP ) was PCR cloned from pLenti CMV GFP Puro (Addgene 658-5) with primers CTCGTCGACCATGGTGAGCAAGGG and CTCGGATCC CGTTACTTGTACAGCTCGT and cloned into hIL8 -pCHD at the SalI and BamHI restriction sites. .. Cloning for bimolecular fluorescence complementation (BiFC) of store operated calcium entry (SOCE) genes lentivirus constructs: N-terminal Venus fragment fused to full-length ORAI1 and C-terminal Venus fused to full-length STIM1 were cut out and amplified from plasmids pcDNA3-Venus-173-N-ORAI1 (a gift from Jin Zhang; Addgene plasmid #87618) and pcDNA3.1-STIM1-Venus-173-C (a gift from Jin Zhang; Addgene plasmid #87619), respectively. .. These two inserts were separately cloned into the pCDH-CMV-Nluc plasmid (a gift from Kazuhiro Oka; Addgene plasmid #73038) in place of the Nanoluciferase (Nluc) reporter using BamH1 and EcoR1 restriction sites to yield two distinct plasmids, each containing a unique fragment for BiFC.

    Bimolecular Fluorescence Complementation Assay:

    Article Title: A shear stress-responsive pathway in monocytes drives cardiopulmonary bypass-induced inflammation via spectrin/RAF1/store-operated calcium entry
    Article Snippet: Enhanced GFP (EGFP) was PCR cloned from pLenti CMV GFP Puro (Addgene 658–5) with primers CTCGTCGACCATGGTGAGCAAGGG and CTCGGATCC CGTTACTTGTACAGCTCGT and cloned into hIL8 -pCHD at the SalI and BamHI restriction sites. .. Cloning for bimolecular fluorescence complementation (BiFC) of store operated calcium entry (SOCE) genes lentivirus constructs: N-terminal Venus’s fragment fused to full-length ORAI1 and C-terminal Venus fused to full-length STIM1 were cut out and amplified from plasmids pcDNA3-Venus-173-N-ORAI1 (a gift from Jin Zhang; Addgene plasmid #87618) and pcDNA3.1-STIM1-Venus-173-C (a gift from Jin Zhang; Addgene plasmid #87619), respectively. .. These two inserts were separately cloned into the pCDH-CMV-Nluc plasmid (a gift from Kazuhiro Oka; Addgene plasmid #73038) in place of the Nanoluciferase (Nluc) reporter using BamH1 and EcoR1 restriction sites to yield two distinct plasmids, each containing a unique fragment for BiFC.

    Article Title: A shear stress-responsive pathway in monocytes drives cardiopulmonary bypass-induced inflammation via spectrin/RAF1/store-operated calcium entry.
    Article Snippet: Enhanced GFP (EGFP) was PCR cloned from pLenti CMV GFP Puro (Addgene 658-5) with primers CTCGTCGACCATGGTGAGCAAGGG and CTCGGATCC CGTTACTTGTACAGCTCGT and cloned into hIL8-pCHD at the SalI and BamHI restriction sites. .. Cloning for bimolecular fluorescence complementation (BiFC) of store operated calcium entry (SOCE) genes lentivirus constructs: N-terminal Venus’s fragment fused to full-length ORAI1 and C-terminal Venus fused to full-length STIM1 were cut out and amplified from plasmids pcDNA3-Venus-173-N-ORAI1 (a gift from Jin Zhang; Addgene plasmid #87618) and pcDNA3.1-STIM1-Venus-173-C (a gift from Jin Zhang; Addgene plasmid #87619), respectively. .. These two inserts were separately cloned into the pCDH-CMV-Nluc plasmid (a gift from Kazuhiro Oka; Addgene plasmid #73038) in place of the Nanoluciferase (Nluc) reporter using BamH1 and EcoR1 restriction sites to yield two distinct plasmids, each containing a unique fragment for BiFC.

    Article Title: Hemodynamic stress activates inflammatory responses and cell death through spectrin-dependent modulation of Store Operated Calcium Entry
    Article Snippet: Enhanced GFP (EGFP ) was PCR cloned from pLenti CMV GFP Puro (Addgene 658-5) with primers CTCGTCGACCATGGTGAGCAAGGG and CTCGGATCC CGTTACTTGTACAGCTCGT and cloned into hIL8 -pCHD at the SalI and BamHI restriction sites. .. Cloning for bimolecular fluorescence complementation (BiFC) of store operated calcium entry (SOCE) genes lentivirus constructs: N-terminal Venus fragment fused to full-length ORAI1 and C-terminal Venus fused to full-length STIM1 were cut out and amplified from plasmids pcDNA3-Venus-173-N-ORAI1 (a gift from Jin Zhang; Addgene plasmid #87618) and pcDNA3.1-STIM1-Venus-173-C (a gift from Jin Zhang; Addgene plasmid #87619), respectively. .. These two inserts were separately cloned into the pCDH-CMV-Nluc plasmid (a gift from Kazuhiro Oka; Addgene plasmid #73038) in place of the Nanoluciferase (Nluc) reporter using BamH1 and EcoR1 restriction sites to yield two distinct plasmids, each containing a unique fragment for BiFC.

    Construct:

    Article Title: A shear stress-responsive pathway in monocytes drives cardiopulmonary bypass-induced inflammation via spectrin/RAF1/store-operated calcium entry
    Article Snippet: Enhanced GFP (EGFP) was PCR cloned from pLenti CMV GFP Puro (Addgene 658–5) with primers CTCGTCGACCATGGTGAGCAAGGG and CTCGGATCC CGTTACTTGTACAGCTCGT and cloned into hIL8 -pCHD at the SalI and BamHI restriction sites. .. Cloning for bimolecular fluorescence complementation (BiFC) of store operated calcium entry (SOCE) genes lentivirus constructs: N-terminal Venus’s fragment fused to full-length ORAI1 and C-terminal Venus fused to full-length STIM1 were cut out and amplified from plasmids pcDNA3-Venus-173-N-ORAI1 (a gift from Jin Zhang; Addgene plasmid #87618) and pcDNA3.1-STIM1-Venus-173-C (a gift from Jin Zhang; Addgene plasmid #87619), respectively. .. These two inserts were separately cloned into the pCDH-CMV-Nluc plasmid (a gift from Kazuhiro Oka; Addgene plasmid #73038) in place of the Nanoluciferase (Nluc) reporter using BamH1 and EcoR1 restriction sites to yield two distinct plasmids, each containing a unique fragment for BiFC.

    Article Title: A shear stress-responsive pathway in monocytes drives cardiopulmonary bypass-induced inflammation via spectrin/RAF1/store-operated calcium entry.
    Article Snippet: Enhanced GFP (EGFP) was PCR cloned from pLenti CMV GFP Puro (Addgene 658-5) with primers CTCGTCGACCATGGTGAGCAAGGG and CTCGGATCC CGTTACTTGTACAGCTCGT and cloned into hIL8-pCHD at the SalI and BamHI restriction sites. .. Cloning for bimolecular fluorescence complementation (BiFC) of store operated calcium entry (SOCE) genes lentivirus constructs: N-terminal Venus’s fragment fused to full-length ORAI1 and C-terminal Venus fused to full-length STIM1 were cut out and amplified from plasmids pcDNA3-Venus-173-N-ORAI1 (a gift from Jin Zhang; Addgene plasmid #87618) and pcDNA3.1-STIM1-Venus-173-C (a gift from Jin Zhang; Addgene plasmid #87619), respectively. .. These two inserts were separately cloned into the pCDH-CMV-Nluc plasmid (a gift from Kazuhiro Oka; Addgene plasmid #73038) in place of the Nanoluciferase (Nluc) reporter using BamH1 and EcoR1 restriction sites to yield two distinct plasmids, each containing a unique fragment for BiFC.

    Article Title: Hemodynamic stress activates inflammatory responses and cell death through spectrin-dependent modulation of Store Operated Calcium Entry
    Article Snippet: Enhanced GFP (EGFP ) was PCR cloned from pLenti CMV GFP Puro (Addgene 658-5) with primers CTCGTCGACCATGGTGAGCAAGGG and CTCGGATCC CGTTACTTGTACAGCTCGT and cloned into hIL8 -pCHD at the SalI and BamHI restriction sites. .. Cloning for bimolecular fluorescence complementation (BiFC) of store operated calcium entry (SOCE) genes lentivirus constructs: N-terminal Venus fragment fused to full-length ORAI1 and C-terminal Venus fused to full-length STIM1 were cut out and amplified from plasmids pcDNA3-Venus-173-N-ORAI1 (a gift from Jin Zhang; Addgene plasmid #87618) and pcDNA3.1-STIM1-Venus-173-C (a gift from Jin Zhang; Addgene plasmid #87619), respectively. .. These two inserts were separately cloned into the pCDH-CMV-Nluc plasmid (a gift from Kazuhiro Oka; Addgene plasmid #73038) in place of the Nanoluciferase (Nluc) reporter using BamH1 and EcoR1 restriction sites to yield two distinct plasmids, each containing a unique fragment for BiFC.

    Amplification:

    Article Title: A shear stress-responsive pathway in monocytes drives cardiopulmonary bypass-induced inflammation via spectrin/RAF1/store-operated calcium entry
    Article Snippet: Enhanced GFP (EGFP) was PCR cloned from pLenti CMV GFP Puro (Addgene 658–5) with primers CTCGTCGACCATGGTGAGCAAGGG and CTCGGATCC CGTTACTTGTACAGCTCGT and cloned into hIL8 -pCHD at the SalI and BamHI restriction sites. .. Cloning for bimolecular fluorescence complementation (BiFC) of store operated calcium entry (SOCE) genes lentivirus constructs: N-terminal Venus’s fragment fused to full-length ORAI1 and C-terminal Venus fused to full-length STIM1 were cut out and amplified from plasmids pcDNA3-Venus-173-N-ORAI1 (a gift from Jin Zhang; Addgene plasmid #87618) and pcDNA3.1-STIM1-Venus-173-C (a gift from Jin Zhang; Addgene plasmid #87619), respectively. .. These two inserts were separately cloned into the pCDH-CMV-Nluc plasmid (a gift from Kazuhiro Oka; Addgene plasmid #73038) in place of the Nanoluciferase (Nluc) reporter using BamH1 and EcoR1 restriction sites to yield two distinct plasmids, each containing a unique fragment for BiFC.

    Article Title: A shear stress-responsive pathway in monocytes drives cardiopulmonary bypass-induced inflammation via spectrin/RAF1/store-operated calcium entry.
    Article Snippet: Enhanced GFP (EGFP) was PCR cloned from pLenti CMV GFP Puro (Addgene 658-5) with primers CTCGTCGACCATGGTGAGCAAGGG and CTCGGATCC CGTTACTTGTACAGCTCGT and cloned into hIL8-pCHD at the SalI and BamHI restriction sites. .. Cloning for bimolecular fluorescence complementation (BiFC) of store operated calcium entry (SOCE) genes lentivirus constructs: N-terminal Venus’s fragment fused to full-length ORAI1 and C-terminal Venus fused to full-length STIM1 were cut out and amplified from plasmids pcDNA3-Venus-173-N-ORAI1 (a gift from Jin Zhang; Addgene plasmid #87618) and pcDNA3.1-STIM1-Venus-173-C (a gift from Jin Zhang; Addgene plasmid #87619), respectively. .. These two inserts were separately cloned into the pCDH-CMV-Nluc plasmid (a gift from Kazuhiro Oka; Addgene plasmid #73038) in place of the Nanoluciferase (Nluc) reporter using BamH1 and EcoR1 restriction sites to yield two distinct plasmids, each containing a unique fragment for BiFC.

    Article Title: Hemodynamic stress activates inflammatory responses and cell death through spectrin-dependent modulation of Store Operated Calcium Entry
    Article Snippet: Enhanced GFP (EGFP ) was PCR cloned from pLenti CMV GFP Puro (Addgene 658-5) with primers CTCGTCGACCATGGTGAGCAAGGG and CTCGGATCC CGTTACTTGTACAGCTCGT and cloned into hIL8 -pCHD at the SalI and BamHI restriction sites. .. Cloning for bimolecular fluorescence complementation (BiFC) of store operated calcium entry (SOCE) genes lentivirus constructs: N-terminal Venus fragment fused to full-length ORAI1 and C-terminal Venus fused to full-length STIM1 were cut out and amplified from plasmids pcDNA3-Venus-173-N-ORAI1 (a gift from Jin Zhang; Addgene plasmid #87618) and pcDNA3.1-STIM1-Venus-173-C (a gift from Jin Zhang; Addgene plasmid #87619), respectively. .. These two inserts were separately cloned into the pCDH-CMV-Nluc plasmid (a gift from Kazuhiro Oka; Addgene plasmid #73038) in place of the Nanoluciferase (Nluc) reporter using BamH1 and EcoR1 restriction sites to yield two distinct plasmids, each containing a unique fragment for BiFC.

    Plasmid Preparation:

    Article Title: A shear stress-responsive pathway in monocytes drives cardiopulmonary bypass-induced inflammation via spectrin/RAF1/store-operated calcium entry
    Article Snippet: Enhanced GFP (EGFP) was PCR cloned from pLenti CMV GFP Puro (Addgene 658–5) with primers CTCGTCGACCATGGTGAGCAAGGG and CTCGGATCC CGTTACTTGTACAGCTCGT and cloned into hIL8 -pCHD at the SalI and BamHI restriction sites. .. Cloning for bimolecular fluorescence complementation (BiFC) of store operated calcium entry (SOCE) genes lentivirus constructs: N-terminal Venus’s fragment fused to full-length ORAI1 and C-terminal Venus fused to full-length STIM1 were cut out and amplified from plasmids pcDNA3-Venus-173-N-ORAI1 (a gift from Jin Zhang; Addgene plasmid #87618) and pcDNA3.1-STIM1-Venus-173-C (a gift from Jin Zhang; Addgene plasmid #87619), respectively. .. These two inserts were separately cloned into the pCDH-CMV-Nluc plasmid (a gift from Kazuhiro Oka; Addgene plasmid #73038) in place of the Nanoluciferase (Nluc) reporter using BamH1 and EcoR1 restriction sites to yield two distinct plasmids, each containing a unique fragment for BiFC.

    Article Title: A shear stress-responsive pathway in monocytes drives cardiopulmonary bypass-induced inflammation via spectrin/RAF1/store-operated calcium entry.
    Article Snippet: Enhanced GFP (EGFP) was PCR cloned from pLenti CMV GFP Puro (Addgene 658-5) with primers CTCGTCGACCATGGTGAGCAAGGG and CTCGGATCC CGTTACTTGTACAGCTCGT and cloned into hIL8-pCHD at the SalI and BamHI restriction sites. .. Cloning for bimolecular fluorescence complementation (BiFC) of store operated calcium entry (SOCE) genes lentivirus constructs: N-terminal Venus’s fragment fused to full-length ORAI1 and C-terminal Venus fused to full-length STIM1 were cut out and amplified from plasmids pcDNA3-Venus-173-N-ORAI1 (a gift from Jin Zhang; Addgene plasmid #87618) and pcDNA3.1-STIM1-Venus-173-C (a gift from Jin Zhang; Addgene plasmid #87619), respectively. .. These two inserts were separately cloned into the pCDH-CMV-Nluc plasmid (a gift from Kazuhiro Oka; Addgene plasmid #73038) in place of the Nanoluciferase (Nluc) reporter using BamH1 and EcoR1 restriction sites to yield two distinct plasmids, each containing a unique fragment for BiFC.

    Article Title: Hemodynamic stress activates inflammatory responses and cell death through spectrin-dependent modulation of Store Operated Calcium Entry
    Article Snippet: Enhanced GFP (EGFP ) was PCR cloned from pLenti CMV GFP Puro (Addgene 658-5) with primers CTCGTCGACCATGGTGAGCAAGGG and CTCGGATCC CGTTACTTGTACAGCTCGT and cloned into hIL8 -pCHD at the SalI and BamHI restriction sites. .. Cloning for bimolecular fluorescence complementation (BiFC) of store operated calcium entry (SOCE) genes lentivirus constructs: N-terminal Venus fragment fused to full-length ORAI1 and C-terminal Venus fused to full-length STIM1 were cut out and amplified from plasmids pcDNA3-Venus-173-N-ORAI1 (a gift from Jin Zhang; Addgene plasmid #87618) and pcDNA3.1-STIM1-Venus-173-C (a gift from Jin Zhang; Addgene plasmid #87619), respectively. .. These two inserts were separately cloned into the pCDH-CMV-Nluc plasmid (a gift from Kazuhiro Oka; Addgene plasmid #73038) in place of the Nanoluciferase (Nluc) reporter using BamH1 and EcoR1 restriction sites to yield two distinct plasmids, each containing a unique fragment for BiFC.

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